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Secondary Antibodies

Secondary antibodies bind to a primary antibody to assist in detection, amplification, sorting, and purification of target antigens, and are used in various biochemical techniques/assays (e.g., ELISA, western blot (WB), immunohistochemistry).
Goat Secondary Antibodies (44,180)
Mouse Secondary Antibodies (14,490)
Rabbit Secondary Antibodies (6,301)
Donkey Secondary Antibodies (4,319)
Rat Secondary Antibodies (1,234)
Sheep Secondary Antibodies (701)
Chicken Secondary Antibodies (403)
Bovine Secondary Antibodies (257)
Pig Secondary Antibodies (76)
Horse Secondary Antibodies (74)
Alpaca Secondary Antibodies (62)
Hamster Secondary Antibodies (57)
Biosimilar Secondary Antibodies (9)
Human Secondary Antibodies (7)
Duck Secondary Antibodies (3)

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Keyword Search:
5,671 – 5,685 of 71,978 results
5,671

Rabbit anti-Goat IgG (H+L) Secondary Antibody, Alkaline Phosphatase (Pre-adsorbed), Novus Biologicals™
SDP

Rabbit Polyclonal Antibody

Content And Storage Store at 4C. After dilution, add an equal volume of glycerol to prevent loss of enzymatic activity and store at -20C. Avoid repeated freeze-thaw cycles.
Target Species Goat
Host Species Rabbit
Conjugate Alkaline Phosphatase (AP)
Applications ELISA,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration 1.50mg/mL
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity purified
Dilution Western Blot 1:200-1:5000, ELISA 1:200-1:5000, Immunohistochemistry 1:500-1:5000, Immunohistochemistry-Paraffin
Classification Polyclonal
Immunogen Purified Goat IgG (H&L)
Primary or Secondary Secondary
Test Specificity Based on IEP, this antibody reacts with heavy gamma chains on goat IgG and light chains on all goat immunoglobulins. This antibody has been pre-adsorbed against human, mouse, and rat IgG.
5,672

Rabbit F(ab')2 anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed), Novus Biologicals™
SDP

Rabbit Polyclonal Antibody

Content And Storage Store at 4C. Do not freeze.
Target Species Goat
Host Species Rabbit
Conjugate Unconjugated
Applications Chemiluminescence Immunoassay (CIA),ELISA,Flow Cytometry,Immunoassay,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunomicroscopy,Western Blot
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration 4.5mg/mL
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity purified
Dilution Immunoassay
Classification Polyclonal
Immunogen Purified Goat IgG (H&L)
Primary or Secondary Secondary
Test Specificity Based on IEP, this antibody reacts with heavy gamma chains on goat IgG and light chains on all goat immunoglobulins. This antibody has been pre-adsorbed against human, mouse, and rat IgG.
5,673

Mouse anti-Human IgG Fc Secondary Antibody (4A10), Novus Biologicals™
SDP

Mouse Monoclonal Antibody

Content And Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Target Species Human
Host Species Mouse
Conjugate Unconjugated
Applications ELISA,Immunocytochemistry/Immunofluorescence,Western Blot
Form Ascites
Isotype IgG1
Antigen IgG Fc
Regulatory Status RUO
Purification Method Ascites
Dilution Western Blot 1:500 - 1:2000, ELISA 1:10000, Immunocytochemistry/Immunofluorescence 1:200 - 1:1000
Classification Monoclonal
Immunogen Purified fusion protein with human IgG(Fc Specific) tag.
Primary or Secondary Secondary
Clone 4A10
5,674

Donkey anti-Rat IgG (H+L) Secondary Antibody, FITC, Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4C. Do not freeze.
Target Species Rat
Host Species Donkey
Conjugate FITC
Applications Flow Cytometry,Immunomicroscopy
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration 1.0mg/mL
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity purified
Dilution Flow Cytometry 1:20-1:2000, Immunomicroscopy 1:20-1:2000
Classification Polyclonal
Immunogen Purified Rat IgG (H&L)
Primary or Secondary Secondary
Test Specificity Based on IEP, this antibody reacts with heavy gamma chains on rat IgG and light chains on all rat immunoglobulins
5,675

Rabbit anti-Rat IgG (H+L) Secondary Antibody, DyLight 800, Novus Biologicals™
SDP

Rabbit Polyclonal Antibody

Content And Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Target Species Rat
Host Species Rabbit
Conjugate DyLight 800
Applications Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence,Western Blot
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration LYOPH
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Western Blot 1:10000-1:25000, Immunocytochemistry/Immunofluorescence 1:5000, Fluorophore-linked immunosorbent assay 1:20000
Classification Polyclonal
Immunogen Rat IgG whole molecule
Reconstitution Reconstitute with 100 ul deionized water (or equivalent).
Primary or Secondary Secondary
Test Specificity This antibody will react with heavy chains of Rat IgG and with light chains of most Rat immunoglobulins.
5,676

Goat anti-Human IgG Fc Secondary Antibody, HRP (Pre-adsorbed), Novus Biologicals™
SDP

Goat Polyclonal Antibody

Content And Storage Store lyophilized antibody at 4°C. Mix reconstituted liquid with an equal volume of glycerol, aliquot contents and freeze at -20°C or below for long term storage.
Target Species Human
Host Species Goat
Conjugate HRP
Applications ELISA,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Isotype IgG
Gene Accession No. O75015, O75015, O75015
Concentration LYOPH
Antigen IgG Fc
Regulatory Status RUO
Purification Method Affinity purified
Dilution Western Blot 1:200-1:5000, ELISA 1:200-1:5000, Immunohistochemistry 1:500-1:5000, Immunohistochemistry-Paraffin
Classification Polyclonal
Immunogen Purified Human IgG, Fc fragment
Reconstitution Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Primary or Secondary Secondary
Test Specificity Based on IEP, this antibody reacts with heavy gamma chains on human IgG. This antibody has been pre-adsorbed against human IgA + IgM, bovine, horse, mouse, and rabbit serum proteins.
5,677

Mouse anti-Human IgG (H+L) Secondary Antibody, HRP, Novus Biologicals™
SDP

Mouse Polyclonal Antibody

Content And Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Target Species Human
Host Species Mouse
Conjugate HRP
Applications ELISA,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Paraffin),KnockDown,Western Blot
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration LYOPH
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Western Blot 1:1000 - 1:5000, ELISA 1:10000 - 1:50000, Immunohistochemistry 1:500 - 1:2500, Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml, Immunohistochemistry-Paraffin 1:500-1:1000, Knockdown Validated
Classification Polyclonal
Immunogen Human IgG whole molecule
Reconstitution Reconstitute with 1.0 ml deionized water (or equivalent).
Primary or Secondary Secondary
Test Specificity This product was prepared from monospecific polyclonal ascites by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum, Human IgG and Human Serum.
5,678

Donkey anti-Goat IgG (H+L) Secondary Antibody, DyLight 488 (Pre-adsorbed), Novus Biologicals™
SDP

Donkey Polyclonal Antibody has been used in 2 publications

Content And Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Target Species Goat
Host Species Donkey
Conjugate DyLight 488
Applications Dot Blot,Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Frozen),Western Blot
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration LYOPH
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Western Blot 1:10000, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1:5000, Immunohistochemistry-Frozen, Dot Blot, Fluorophore-linked immunosorbent assay 1:20000
Classification Polyclonal
Immunogen Goat IgG whole molecule
Reconstitution Reconstitute with 100 ul deionized water (or equivalent).
Primary or Secondary Secondary
Test Specificity This antibody was pre-adsorbed against Chicken, Guinea Pig, Hamster, Horse, Mouse, Rabbit, and Rat Serum Proteins. No reaction was observed against Chicken, Guinea Pig, Hamster, Horse, Mouse, Rabbit and Rat Serum Proteins. This antibody will react with heavy chains of Goat IgG and with light chains of most Goat immunoglobulins.
5,679

Rabbit anti-Goat IgG (H+L) Secondary Antibody, Alkaline Phosphatase (Pre-adsorbed), Novus Biologicals™
SDP

Rabbit Polyclonal Antibody

Content And Storage Store at 4C. Do not freeze.
Target Species Goat
Host Species Rabbit
Conjugate Alkaline Phosphatase (AP)
Applications ELISA,Immunohistochemistry,Western Blot
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration 1 mg/mL
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Western Blot 1:500 - 1:2000, ELISA 1:10000 - 1:20000, Immunohistochemistry 1:200 - 1:1000
Classification Polyclonal
Immunogen Goat IgG whole molecule
Primary or Secondary Secondary
Test Specificity This antibody was pre-adsorbed against Human Serum Proteins. No reaction was observed against Human Serum Proteins.
5,680

Rabbit anti-Canine IgG Fc Secondary Antibody, Novus Biologicals™
SDP

Rabbit Polyclonal Antibody

Content And Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Target Species Canine
Host Species Rabbit
Conjugate Unconjugated
Applications Dot Blot,ELISA,Immunohistochemistry,Western Blot
Isotype IgG
Concentration 2 mg/mL
Antigen IgG Fc
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Western Blot 1:2000 - 1:10000, ELISA 1:20000 - 1:100000, Immunohistochemistry 1:1000 - 1:5000, Dot Blot
Classification Polyclonal
Immunogen Dog IgG F(c) fragment
Primary or Secondary Secondary
Test Specificity No reaction was observed against Dog IgG F(ab).
5,681

Goat anti-Mouse IgG/IgM (H+L) Secondary Antibody, HRP, Novus Biologicals™
SDP

Goat Polyclonal Antibody

Content And Storage Store lyophilized antibody at 4°C. Mix reconstituted liquid with an equal volume of glycerol, aliquot contents and freeze at -20°C or below for long term storage.
Target Species Mouse
Host Species Goat
Conjugate HRP
Applications ELISA,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Isotype IgG
Concentration LYOPH
Antigen IgG/IgM (H+L)
Regulatory Status RUO
Purification Method Affinity purified
Dilution Western Blot 1:200-1:5000, ELISA 1:200-1:5000, Immunohistochemistry 1:500-1:5000, Immunohistochemistry-Paraffin
Classification Polyclonal
Immunogen Purified Mouse IgG + IgM
Reconstitution Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Primary or Secondary Secondary
Test Specificity Based on IEP, this antibody reacts with heavy chains on mouse IgG + IgM and light chains on all mouse immunoglobulins
5,682

Mouse anti-Human IgG Fc Secondary Antibody (EM-07), HRP, Novus Biologicals™
SDP

Mouse Monoclonal Antibody

Content And Storage Store at 4C in the dark.
Target Species Human
Host Species Mouse
Conjugate HRP
Applications ELISA,Western Blot
Form Purified
Isotype IgG1
Concentration 1 mg/ml
Antigen IgG Fc
Regulatory Status RUO
Purification Method Protein A or G purified
Dilution Western Blot 1:1000, ELISA 1:2000
Classification Monoclonal
Immunogen Fusion protein of human IgG Fc fragment.
Primary or Secondary Secondary
Test Specificity This secondary antibody reacts with Fc part of human IgG heavy chain and with isolated Fc fragments.
Clone EM-07
5,683

Goat F(ab')2 anti-Rabbit IgG F(ab')2 Secondary Antibody, Biotin (Pre-adsorbed), Novus Biologicals™
SDP

Goat Polyclonal Antibody

Content And Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Target Species Rabbit
Host Species Goat
Conjugate Biotin
Applications ELISA,Immunohistochemistry,Western Blot
Isotype IgG
Concentration 0.5 mg/mL
Antigen IgG F(ab')2
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Western Blot 1:2000 - 1:10000, ELISA 1:20000 - 1:100000, Immunohistochemistry 1:1000 - 1:5000
Gene Alias immunoglobulin heavy constant gamma 1 (G1m marker)
Classification Polyclonal
Immunogen Rabbit IgG F(ab')2 fragment
Primary or Secondary Secondary
Test Specificity No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rabbit IgG F(c) or Bovine, Horse, Human, Mouse, Rat and Sheep Serum Proteins.
5,684

Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alkaline Phosphatase (Pre-adsorbed), Novus Biologicals™
SDP

Goat Polyclonal Antibody

Content And Storage Store at 4C. Do not freeze.
Target Species Rabbit
Host Species Goat
Conjugate Alkaline Phosphatase (AP)
Applications ELISA,Immunohistochemistry,Western Blot
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Antigen IgG (H+L)
Gene Symbols IGHG
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Western Blot 1:500 - 1:2500, ELISA 1:10000 - 1:50000, Immunohistochemistry 1:200 - 1:1000
Classification Polyclonal
Immunogen Rabbit IgG whole molecule
Primary or Secondary Secondary
Test Specificity This antibody was pre-adsorbed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat, and Sheep Serum Proteins. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.
5,685

Goat F(ab')2 anti-Mouse IgG Fc Secondary Antibody, HRP (Pre-adsorbed), Novus Biologicals™
SDP

Goat Polyclonal Antibody

Content And Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Target Species Mouse
Host Species Goat
Conjugate HRP
Applications ELISA,Immunohistochemistry,Western Blot
Isotype IgG
Antigen IgG Fc
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Western Blot 1:500 - 1:2000, ELISA 1:100000, Immunohistochemistry 1:250 - 1:1000
Classification Polyclonal
Immunogen Mouse IgG F(c) fragment
Primary or Secondary Secondary
Test Specificity No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Mouse IgG F(ab) or Bovine, Horse, and Human Serum Proteins.